genepix pro 6.0 microarray image analysis software Search Results


trizol  (Thermo Fisher)
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Thermo Fisher trizol
Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genepix pro 6.0  (Agilent technologies)
90
Agilent technologies genepix pro 6.0
Genepix Pro 6.0, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genepix 4000  (Amersham Pharmacia Biotech Ltd)
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Amersham Pharmacia Biotech Ltd genepix 4000
Genepix 4000, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genepix pro 6 0  (Danaher Inc)
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Danaher Inc genepix pro 6 0
Genepix Pro 6 0, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microarray scanner  (Agilent technologies)
90
Agilent technologies microarray scanner
Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microarray scanner  (Danaher Inc)
86
Danaher Inc microarray scanner
Microarray Scanner, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wash buffer kit  (Qiagen)
90
Qiagen wash buffer kit
Wash Buffer Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genepix pro  (Danaher Inc)
86
Danaher Inc genepix pro
Genepix Pro, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spotfire decision site microarray analysis  (TIBCO)
90
TIBCO spotfire decision site microarray analysis
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Spotfire Decision Site Microarray Analysis, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genepix 6.0  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc genepix 6.0
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Genepix 6.0, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microarray slide  (Corning Life Sciences)
90
Corning Life Sciences microarray slide
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Microarray Slide, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microarray slide - by Bioz Stars, 2026-04
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surescan microarray scanner  (Agilent technologies)
90
Agilent technologies surescan microarray scanner
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Surescan Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.

Journal: PLoS ONE

Article Title: Role of TNF in the Altered Interaction of Dormant Mycobacterium tuberculosis with Host Macrophages

doi: 10.1371/journal.pone.0095220

Figure Lengend Snippet: ( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.

Article Snippet: Briefly, we used GenePix 4000B to scan chip images, GenePix Pro 6.0 to acquire raw data and Spotfire Decision Site for Microarray Analysis (TIBCO-Spotfire Inc) to perform data analysis using protocols that have been developed by us .

Techniques: Microarray, Infection, Expressing, Isolation, Quantitative RT-PCR, Cytokine Assay, Confocal Microscopy, Derivative Assay