genepix pro 6.0 microarray image analysis software Search Results


genepix pro 6 0  (Molecular Devices LLC)
94
Molecular Devices LLC genepix pro 6 0
Genepix Pro 6 0, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genepix pro 6 0/product/Molecular Devices LLC
Average 94 stars, based on 1 article reviews
genepix pro 6 0 - by Bioz Stars, 2026-06
94/100 stars
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spotfire decision site microarray analysis  (TIBCO)
90
TIBCO spotfire decision site microarray analysis
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Spotfire Decision Site Microarray Analysis, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spotfire decision site microarray analysis - by Bioz Stars, 2026-06
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microarray slide  (Corning Life Sciences)
90
Corning Life Sciences microarray slide
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Microarray Slide, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microarray slide/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
microarray slide - by Bioz Stars, 2026-06
90/100 stars
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luxscan 10k-a  (CapitalBio Corporation)
90
CapitalBio Corporation luxscan 10k-a
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Luxscan 10k A, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luxscan 10k-a/product/CapitalBio Corporation
Average 90 stars, based on 1 article reviews
luxscan 10k-a - by Bioz Stars, 2026-06
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resolver 6.0  (Rosetta Biosoftware)
90
Rosetta Biosoftware resolver 6.0
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Resolver 6.0, supplied by Rosetta Biosoftware, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/resolver 6.0/product/Rosetta Biosoftware
Average 90 stars, based on 1 article reviews
resolver 6.0 - by Bioz Stars, 2026-06
90/100 stars
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cy3 fluorescent dye marker  (Arraystar inc)
90
Arraystar inc cy3 fluorescent dye marker
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Cy3 Fluorescent Dye Marker, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cy3 fluorescent dye marker/product/Arraystar inc
Average 90 stars, based on 1 article reviews
cy3 fluorescent dye marker - by Bioz Stars, 2026-06
90/100 stars
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luxscan microarray scanner  (CapitalBio Corporation)
90
CapitalBio Corporation luxscan microarray scanner
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Luxscan Microarray Scanner, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luxscan microarray scanner/product/CapitalBio Corporation
Average 90 stars, based on 1 article reviews
luxscan microarray scanner - by Bioz Stars, 2026-06
90/100 stars
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sensospot ® fluorescence microarray  (Sensovation ag)
90
Sensovation ag sensospot ® fluorescence microarray
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Sensospot ® Fluorescence Microarray, supplied by Sensovation ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sensospot ® fluorescence microarray - by Bioz Stars, 2026-06
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genepix pro 6.0 microarray image analysis software  (MDS Inc)
90
MDS Inc genepix pro 6.0 microarray image analysis software
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Genepix Pro 6.0 Microarray Image Analysis Software, supplied by MDS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genepix pro 6.0 microarray image analysis software/product/MDS Inc
Average 90 stars, based on 1 article reviews
genepix pro 6.0 microarray image analysis software - by Bioz Stars, 2026-06
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axon genepix pro  (Bucher Biotec AG)
90
Bucher Biotec AG axon genepix pro
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Axon Genepix Pro, supplied by Bucher Biotec AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axon genepix pro/product/Bucher Biotec AG
Average 90 stars, based on 1 article reviews
axon genepix pro - by Bioz Stars, 2026-06
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axon 4100a fluorescence scanner  (Bucher Biotec AG)
90
Bucher Biotec AG axon 4100a fluorescence scanner
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Axon 4100a Fluorescence Scanner, supplied by Bucher Biotec AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axon 4100a fluorescence scanner - by Bioz Stars, 2026-06
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blocking solution  (CANDOR Bioscience)
90
CANDOR Bioscience blocking solution
( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
Blocking Solution, supplied by CANDOR Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.

Journal: PLoS ONE

Article Title: Role of TNF in the Altered Interaction of Dormant Mycobacterium tuberculosis with Host Macrophages

doi: 10.1371/journal.pone.0095220

Figure Lengend Snippet: ( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.

Article Snippet: Briefly, we used GenePix 4000B to scan chip images, GenePix Pro 6.0 to acquire raw data and Spotfire Decision Site for Microarray Analysis (TIBCO-Spotfire Inc) to perform data analysis using protocols that have been developed by us .

Techniques: Microarray, Infection, Expressing, Isolation, Quantitative RT-PCR, Cytokine Assay, Confocal Microscopy, Derivative Assay